Function of the genetic element 'Mona' associated with fungicide resistance in Monilinia fructicola.

ABSTRACT: The genetic element 'Mona' has been shown previously to be associated with resistance to demethylation inhibitors (DMIs) in Monilinia fructicola. In this study, the promoter activity of the 'Mona' element was demonstrated genetically and the activity was narrowed down to a 20-bp active region through a series of deletions. 'Mona' knockout transformants (ΔMona) were generated from DMI-resistant isolate Bmpc7, and EC₅₀ values and expression of the MfCYP51 gene were found to be reduced in transformants compared with the parental isolate. When the 'Mona' element was inserted into the upstream region of the MfCYP51 gene of the DMI-sensitive isolate HG3, the EC₅₀ values and expression of the MfCYP51 gene increased in the transformants compared with the parental sensitive is

SUBMITTER: Chen S 

PROVIDER: S-EPMC6638277 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature