Identification and functional analysis of Penicillium digitatum genes putatively involved in virulence towards citrus fruit.

ABSTRACT: The fungus Penicillium digitatum, the causal agent of green mould rot, is the most destructive post-harvest pathogen of citrus fruit in Mediterranean regions. In order to identify P. digitatum genes up-regulated during the infection of oranges that may constitute putative virulence factors, we followed a polymerase chain reaction (PCR)-based suppression subtractive hybridization and cDNA macroarray hybridization approach. The origin of expressed sequence tags (ESTs) was determined by comparison against the available genome sequences of both organisms. Genes coding for fungal proteases and plant cell wall-degrading enzymes represent the largest categories in the subtracted cDNA library. Northern blot analysis of a selection of P. digitatum genes, including those coding for proteases, cell w

SUBMITTER: Lopez-Perez M 

PROVIDER: S-EPMC6638479 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature