A functional genomics approach to dissect the mode of action of the Stagonospora nodorum effector protein SnToxA in wheat.

ABSTRACT: In this study, proteomics and metabolomics were used to study the wheat response to exposure to the SnToxA effector protein secreted by the fungal pathogen Stagonospora nodorum during infection. Ninety-one different acidic and basic proteins and 101 metabolites were differentially abundant when comparing SnToxA- and control-treated wheat leaves during a 72-h time course. Proteins involved in photosynthesis were observed to increase marginally initially after exposure, before decreasing rapidly and significantly. Proteins and metabolites associated with the detoxification of reactive oxygen species in the chloroplast were also differentially abundant during SnToxA exposure, implying that the disruption of photosynthesis causes the rapid accumulation of chloroplastic reactive oxygen species.

SUBMITTER: Vincent D 

PROVIDER: S-EPMC6638714 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature