Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli.

ABSTRACT: Polyketides are a class of specialised metabolites synthesised by both eukaryotes and prokaryotes. These chemically and structurally diverse molecules are heavily used in the clinic and include frontline antimicrobial and anticancer drugs such as erythromycin and doxorubicin. To replenish the clinicians' diminishing arsenal of bioactive molecules, a promising strategy aims at transferring polyketide biosynthetic pathways from their native producers into the biotechnologically desirable host Escherichia coli. This approach has been successful for type I modular polyketide synthases (PKSs); however, despite more than 3 decades of research, the large and important group of type II PKSs has until now been elusive in E. coli. Here, we report on a versatile polyketide biosynthesis pipeline, base

SUBMITTER: Cummings M 

PROVIDER: S-EPMC6638757 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature