Identification of candidate effector genes in the transcriptome of the rice root knot nematode Meloidogyne graminicola.

ABSTRACT: Plant-parasitic nematodes secrete so-called effectors into their host plant which are able to suppress the plant's defence responses, alter plant signalling pathways and, in the case of root knot nematodes, induce the formation of giant cells. Putative effectors have been successfully identified by genomics, transcriptomics and proteomics approaches. In this study, we investigated the transcriptome of the rice root knot nematode Meloidogyne graminicola by 454 sequencing of second-stage juveniles as well as mRNA-seq of rice infected tissue. Over 350 000 reads derived from M. graminicola preparasitic juveniles were assembled, annotated and checked for homologues in different databases. From infected rice tissue, 1.4% of all reads generated were identified as being derived from the nematode.

SUBMITTER: Haegeman A 

PROVIDER: S-EPMC6638898 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature