Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c.

ABSTRACT: A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contributes to the aggressiveness of the fungus. This result suggests that CSEP0055 is involved in the suppression of plant defence. A yeast two-hybrid screen indicated that CSEP0055 interacts with members of the barley pathogenesis-related protein families, PR1 and PR17. Interaction with PR17c was confirmed by bimolecular fluorescence complementation analyses. Down-regulation and over-expression of PR17c in epidermal cells of barley confirmed that this protein is important for penet

SUBMITTER: Zhang WJ 

PROVIDER: S-EPMC6638906 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature