A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking.

ABSTRACT: Kv4.2 voltage-gated K⁺ channel subunits, the primary source of the somatodendritic A-type K⁺ current in CA1 pyramidal neurons of the hippocampus, play important roles in regulating dendritic excitability and plasticity. To better study the trafficking and subcellular distribution of Kv4.2, we created and characterized a novel Kv4.2 construct encoding a bungarotoxin binding site in the extracellular S3-S4 linker region of the α-subunit. When expressed, this construct can be visualized in living cells after staining with rhodamine-conjugated bungarotoxin. We validated the utility of this construct by visualizing the spontaneous internalization and insertion of Kv4.2 in HEK 293T cells. We further report that Kv4.2 colocalized with several endosome markers in HEK 293T cel

SUBMITTER: Tabor GT 

PROVIDER: S-EPMC6639039 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature