Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport.

ABSTRACT: Localization of RNAs to various subcellular destinations is a widely used mechanism that regulates a large proportion of transcripts in polarized cells. In many cases, such localized transcripts mediate spatial control of gene expression by being translationally silent while in transit and locally activated at their destination. Here, we investigate the translation of RNAs localized at dynamic cellular protrusions of human and mouse, migrating, mesenchymal cells. In contrast to the model described above, we find that protrusion-localized RNAs are not locally activated solely at protrusions, but can be translated with similar efficiency in both internal and peripheral locations. Interestingly, protrusion-localized RNAs are translated at extending protrusions, they become translationally sil

SUBMITTER: Moissoglu K 

PROVIDER: S-EPMC6639073 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature