Thermoregulation via Temperature-Dependent PGD₂ Production in Mouse Preoptic Area.

ABSTRACT: Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD₂), we further explored its role in thermoregulation. Our study revealed

SUBMITTER: Wang TA 

PROVIDER: S-EPMC6639135 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature