Microextrusion printing cell-laden networks of type I collagen with patterned fiber alignment and geometry.

ABSTRACT: Type I collagen self-assembles into three-dimensional (3D) fibrous networks. These dynamic viscoelastic materials can be remodeled in response to mechanical and chemical signals to form anisotropic networks, the structure of which influences tissue development, homeostasis, and disease progression. Conventional approaches for fabricating anisotropic networks of type I collagen are often limited to unidirectional fiber alignment over small areas. Here, we describe a new approach for engineering cell-laden networks of aligned type I collagen fibers using 3D microextrusion printing of a collagen-Matrigel ink. We demonstrate hierarchical control of 3D-printed collagen with the ability to spatially pattern collagen fiber alignment and geometry. Our data suggest that collagen alignment results f

SUBMITTER: Nerger BA 

PROVIDER: S-EPMC6639139 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature