The Major Hurdle for Effective Baculovirus Transduction into Mammalian Cells Is Passing Early Endosomes.

ABSTRACT: Baculoviruses, although they infect insects in nature, can transduce a wide variety of mammalian cells and are therefore promising gene therapy vectors. However, baculovirus transduction into many mammalian cells is very inefficient, and the limiting stages and factors remain unknown. An important finding is that a short-duration trigger with low pH can significantly enhance virus transduction efficiency, but the mechanism is poorly understood. Herein, we performed a detailed comparative study on entry mechanisms of the prototypical baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) into insect and mammalian cells. The results showed that AcMNPV could be internalized into mammalian cells efficiently, but fusion in early endosomes (EEs) appeared to be the major obstac

SUBMITTER: Hu L 

PROVIDER: S-EPMC6639295 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature