Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging.

ABSTRACT: The intestinal epithelium undergoes constant regeneration driven by intestinal stem cells. How old age affects the transcriptome in this highly dynamic tissue is an important, but poorly explored question. Using transcriptomics on sorted intestinal stem cells and adult enterocytes, we identified candidate genes, which change expression on aging. Further validation of these on intestinal epithelium of multiple middle-aged versus old-aged mice highlighted the consistent up-regulation of the expression of the gene encoding chemokine receptor Ccr2, a mediator of inflammation and several disease processes. We observed also increased expression of Strc, coding for stereocilin, and dramatically decreased expression of Rps4l, coding for a ribosome subunit. Ccr2 and Rps4l are located close to the t

SUBMITTER: Kazakevych J 

PROVIDER: S-EPMC6639340 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature