The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice.

ABSTRACT:

Background

The heterotrimeric G protein β subunit RGB1 plays an important role in plant growth and development. However, the molecular mechanisms underlying the regulation of rice growth by RGB1 remain elusive.

Results

Here, the rgb1 mutants rgb1-1 (+ 1 bp), rgb1-2 (- 1 bp), and rgb1-3 (- 11 bp) were isolated using the CRISPR/Cas9 system, and they were arrested at 1 day after germination and ultimately exhibited seedling lethality. The dynamic anatomical characteristics of the embryos of the rgb1 seedlings and WT during early postgermination and according to TUNEL assays showed that the suppressed growth of the rgb1 mutants was caused by cell death. In addition to the limited shoot and root development, the development of the embryo shoot-root axis was suppressed in the rgb1

SUBMITTER: Gao Y 

PROVIDER: S-EPMC6639528 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature