Screening the ToxCast Phase 1 Chemical Library for Inhibition of Deiodinase Type 1 Activity.

ABSTRACT: Thyroid hormone (TH) homeostasis is dependent upon coordination of multiple key events including iodide uptake, hormone synthesis, metabolism, and elimination, to maintain proper TH signaling. Deiodinase enzymes catalyze iodide release from THs to interconvert THs between active and inactive forms, and are integral to hormone metabolism. The activity of deiodinases has been identified as an important endpoint to include in the context of screening chemicals for TH disruption. To begin to address the potential for chemicals to inhibit these enzymes an adenovirus expression system was used to produce human deiodinase type 1 (DIO1) enzyme, established robust assay parameters for nonradioactive determination of iodide release by the Sandell-Kolthoff method, and employed a 96-well plate format

SUBMITTER: Hornung MW 

PROVIDER: S-EPMC6639810 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature