Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation.

ABSTRACT:

Background

Molecular markers for prostate cancer (PCa) are required to improve the early definition of patient outcomes. Atypically large extracellular vesicles (EVs), referred as "Large Oncosomes" (LO), have been identified in highly migratory and invasive PCa cells. We recently developed and characterized the DU145R80 subline, selected from parental DU145 cells as resistant to inhibitors of mevalonate pathway. DU145R80 showed different proteomic profile compared to parental DU145 cells, along with altered cytoskeleton dynamics and a more aggressive phenotype.

Methods

Immunofluorescence staining and western blotting were used to identify blebbing and EVs protein cargo. EVs, purified by gradient ultra-centrifugations, were analyzed by tunable resistive pulse sensing and mult

SUBMITTER: Ciardiello C 

PROVIDER: S-EPMC6639931 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature