Spatial UBE2N protein expression indicates genomic instability in colorectal cancers.

ABSTRACT:

Background

One major hallmark of colorectal cancers (CRC) is genomic instability with its contribution to tumor heterogeneity and therapy resistance. To facilitate the investigation of intra-sample phenotypes and the de novo identification of tumor sub-populations, imaging mass spectrometry (IMS) provides a powerful technique to elucidate the spatial distribution patterns of peptides and proteins in tissue sections.

Methods

In the present study, we analyzed an in-house compiled tissue microarray (n = 60) comprising CRCs and control tissues by IMS. After obtaining protein profiles through direct analysis of tissue sections, two validation sets were used for immunohistochemical evaluation.

Results

A total of 28 m/z values in the mass range 800-3500 Da distinguished eupl

SUBMITTER: Gemoll T 

PROVIDER: S-EPMC6639966 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature