Unbiased phenotypic identification of functionally distinct hematopoietic progenitors.

ABSTRACT:

Background

Hematopoiesis is a model-system for studying cellular development and differentiation. Phenotypic and functional characterization of hematopoietic progenitors has significantly aided our understanding of the mechanisms that govern fate choice, lineage specification and maturity. Methods for progenitor isolation have historically relied on complex flow-cytometric strategies based on nested, arbitrary gates within defined panels of immunophenotypic markers. The resulted populations are then functionally assessed, although functional homogeneity or absolute linkage between function and phenotype is not always achieved, thus distorting our view on progenitor biology.

Method

In this study, we present a protocol for unbiased phenotypic identification and functional char

SUBMITTER: Georgolopoulos G 

PROVIDER: S-EPMC6639971 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature