Expression profiling and mapping of defence response genes associated with the barley-Pyrenophora teres incompatible interaction.

ABSTRACT: Barley net- and spot-form of net blotch disease are caused by two formae of the hemibiotrophic fungus Pyrenophora teres (P. t. f. teres and P. t. f. maculata). In the present study, suppression subtractive hybridization (SSH) was used in combination with quantitative real-time reverse transcriptase PCR to identify and profile the expression of defence response (DR) genes in the early stages of both barley-P. teres incompatible and compatible interactions. From a pool of 307 unique gene transcripts identified by SSH, 45 candidate DR genes were selected for temporal expression profiling in infected leaf epidermis. Differential expression profiles were observed for 28 of the selected candidates, which were grouped into clusters depending on their expression profiles within the first 48 h afte

SUBMITTER: Bogacki P 

PROVIDER: S-EPMC6640259 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature