Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis.

ABSTRACT: Plant parasitic nematodes infect roots and trigger the formation of specialized feeding sites by substantial reprogramming of the developmental process of root cells. In this article, we describe the dynamic changes in the tomato root transcriptome during early interactions with the potato cyst nematode Globodera rostochiensis. Using amplified fragment length polymorphism-based mRNA fingerprinting (cDNA-AFLP), we monitored 17 600 transcript-derived fragments (TDFs) in infected and uninfected tomato roots, 1-14 days after inoculation with nematode larvae. Six hundred and twenty-four TDFs (3.5%) showed significant differential expression on nematode infection. We employed GenEST, a computer program which links gene expression profiles generated by cDNA-AFLP and databases of cDNA sequences, t

SUBMITTER: Swiecicka M 

PROVIDER: S-EPMC6640267 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature