Rapid sequencing of MRSA direct from clinical plates in a routine microbiology laboratory.

ABSTRACT:

Background

Routine sequencing of MRSA could bring about significant improvements to outbreak detection and investigation. Sequencing is commonly performed using DNA extracted from a pure culture, but overcoming the delay associated with this step could reduce the time to infection control interventions.

Objectives

To develop and evaluate rapid sequencing of MRSA using primary clinical cultures.

Methods

Patients with samples submitted to the clinical laboratory at the Cambridge University Hospitals NHS Foundation Trust from which MRSA was isolated were identified, the routine laboratory culture plates obtained and DNA extraction and sequencing performed.

Results

An evaluation of routine MRSA cultures from 30 patients demonstrated that direct sequencing from bact

SUBMITTER: Blane B 

PROVIDER: S-EPMC6640301 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature