Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici by large-scale transposon tagging.

ABSTRACT: Forward genetic screens are efficient tools for the dissection of complex biological processes, such as fungal pathogenicity. A transposon tagging system was developed in the vascular wilt fungus Fusarium oxysporum f. sp. lycopersici by inserting the novel modified impala element imp160::gfp upstream of the Aspergillus nidulans niaD gene, followed by transactivation with a constitutively expressed transposase. A collection of 2072 Nia(+) revertants was obtained from reporter strain T12 and screened for alterations in virulence, using a rapid assay for invasive growth on apple slices. Seven strains exhibited reduced virulence on both apple slices and intact tomato plants. Five of these were true revertants showing the re-insertion of imp160::gfp within or upstream of predicted coding region

SUBMITTER: Lopez-Berges MS 

PROVIDER: S-EPMC6640436 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature