Impact of Acetylated and Non-Acetylated Fucose Analogues on IgG Glycosylation.

ABSTRACT: The biological activity of therapeutic antibodies is highly influenced by their glycosylation profile. A valuable method for increasing the cytotoxic efficacy of antibodies, which are used, for example, in cancer treatment, is the reduction of core fucosylation, as this enhances the elimination of target cells through antibody-dependent cell-mediated cytotoxicity. Development of fucose analogues is currently the most promising strategy to reduce core fucosylation without cell line engineering. Since peracetylated sugars display enhanced cell permeability over the highly polar free hydroxy sugars, this work sought to compare the efficacy of peracetylated sugars to their unprotected forms. Two potent fucose analogues, 2-deoxy-2-fluorofucose and 5-alkynylfucose, and their acetylated forms wer

SUBMITTER: Zimmermann M 

PROVIDER: S-EPMC6640710 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature