Profiling microRNA expression in murine bone healing and non-union formation: Role of miR-140 during the early stage of bone healing.

ABSTRACT: Although cellular and molecular mechanisms during the course of bone healing have been thoroughly investigated, the regulation of gene expression by microRNA during bone regeneration is still poorly understood. We hypothesized that nonunion formation is associated with different microRNA expression patterns and that target proteins of these microRNAs are differently expressed in callus tissue of nonunions compared to physiologically healing bones. In a well-established femoral osteotomy model in CD-1 mice osteotomies were induced which result either in healing or in nonunion formation. MicroRNA and target protein expression was evaluated by microarray, quantitative real-time polymerase chain reaction (qrt-PCR) and Western blot. Microarray analyses demonstrated 44 microRNAs to be relevant f

SUBMITTER: Orth M 

PROVIDER: S-EPMC6641081 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature