Pancreatic ductal cells may have a negative effect on human islet transplantation.

ABSTRACT:

Aim

To evaluate the effect of pancreatic ductal cells on experimental human islet transplantation.

Materials and methods

Isolated islets were additionally purified by handpicking. Ductal cells were purified by magnetic cell sorting and then clustered into ductal pancreatospheres (DPS). Islets, DPS, and islets + DPS (100 islets + 75 DPS, or 100 islets + 200 DPS) were cultured and glucose-stimulated insulin secretion, β-cell apoptosis, and gene expression was determined. Islets and islets + DPS preparations (800 islets + 600 DPS) were transplanted to streptozotocin-treated immunodeficient mice and glycemia, graft morphometry, and gene expression were determined.

Results

Insulin stimulation index was higher in islets than in islets co-cultured with DPS (5.59 ± 0.93 vs 4.

SUBMITTER: Marin-Canas S 

PROVIDER: S-EPMC6641198 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature