Restriction Enzymes as a Target for DNA-Based Sensing and Structural Rearrangement.

ABSTRACT: DNA nanostructures have been shown viable for the creation of complex logic-enabled sensing motifs. To date, most of these types of devices have been limited to the interaction with strictly DNA-type inputs. Restriction endonuclease represents a class of enzyme with endogenous specificity to DNA, and we hypothesize that these can be integrated with a DNA structure for use as inputs to trigger structural transformation and structural rearrangement. In this work, we reconfigured a three-arm DNA switch, which utilizes a cyclic Förster resonance energy transfer interaction between three dyes to produce complex output for the detection of three separate input regions to respond to restriction endonucleases, and investigated the efficacy of the enzyme targets. We demonstrate the ability to use t

SUBMITTER: Buckhout-White S 

PROVIDER: S-EPMC6641459 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature