Cleaning Up Mechanistic Debris Generated by Twister Ribozymes Using Computational RNA Enzymology.

ABSTRACT: The catalytic properties of RNA have been a subject of fascination and intense research since their discovery over 30 years ago. Very recently, several classes of nucleolytic ribozymes have emerged and been characterized structurally. Among these, the twister ribozyme has been center-stage, and a topic of debate about its architecture and mechanism owing to conflicting interpretations of different crystal structures, and in some cases conflicting interpretations of the same functional data. In the present work, we attempt to clean up the mechanistic "debris" generated by twister ribozymes using a comprehensive computational RNA enzymology approach aimed to provide a unified interpretation of existing structural and functional data. Simulations in the crystalline environment and in solution

SUBMITTER: Gaines CS 

PROVIDER: S-EPMC6641568 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature