Placental effects and transfer of sildenafil in healthy and preeclamptic conditions.

ABSTRACT:

Background

The phosphodiesterase-5 inhibitor (PDE5) sildenafil has emerged as a promising treatment for preeclampsia (PE). However, a sildenafil trial was recently halted due to lack of effect and increased neonatal morbidity.

Methods

Ex vivo dual-sided perfusion of an isolated cotyledon and wire-myography on chorionic plate arteries were performed to study the effects of sildenafil and the non-selective PDE inhibitor vinpocetine on the response to the NO donor sodium nitroprusside (SNP) under healthy and PE conditions. Ex vivo perfusion was also used to study placental transfer of sildenafil in 6 healthy and 2 PE placentas. Furthermore, placental mRNA and protein levels of eNOS, iNOS, PDE5 and PDE1 were quantified.

Findings

Sildenafil and vinpocetine significantly en

SUBMITTER: Hitzerd E 

PROVIDER: S-EPMC6642075 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature