Transcriptomic analysis of enteropathy in Zambian children with severe acute malnutrition.

ABSTRACT:

Background

Children with severe acute malnutrition (SAM), with or without diarrhoea, often have enteropathy, but there are few molecular data to guide development of new therapies. We set out to determine whether SAM enteropathy is characterised by specific transcriptional changes which might improve understanding or help identify new treatments.

Methods

We collected intestinal biopsies from children with SAM and persistent diarrhoea. mRNA was extracted from biopsies, sequenced, and subjected to a progressive set of complementary analytical approaches: NOIseq, Gene Set Enrichment Analysis (GSEA), and correlation analysis of phenotypic data with gene expression.

Findings

Transcriptomic profiles were generated for biopsy sets from 27 children of both sexes, under 2 year

SUBMITTER: Chama M 

PROVIDER: S-EPMC6642221 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature