Translational assessment of a genetic engineering methodology to improve islet function for transplantation.

ABSTRACT:

Background

The functional quality of insulin-secreting islet beta cells is a major factor determining the outcome of clinical transplantations for diabetes. It is therefore of importance to develop methodological strategies aiming at optimizing islet cell function prior to transplantation. In this study we propose a synthetic biology approach to genetically engineer cellular signalling pathways in islet cells.

Methods

We established a novel procedure to modify islet beta cell function by combining adenovirus-mediated transduction with reaggregation of islet cells into pseudoislets. As a proof-of-concept for the genetic engineering of islets prior to transplantation, this methodology was applied to increase the expression of the V1b receptor specifically in insulin-secreting

SUBMITTER: van Krieken PP 

PROVIDER: S-EPMC6642289 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature