Predicting microbial growth in a mixed culture from growth curve data.

ABSTRACT: Determining the fitness of specific microbial genotypes has extensive application in microbial genetics, evolution, and biotechnology. While estimates from growth curves are simple and allow high throughput, they are inaccurate and do not account for interactions between costs and benefits accruing over different parts of a growth cycle. For this reason, pairwise competition experiments are the current "gold standard" for accurate estimation of fitness. However, competition experiments require distinct markers, making them difficult to perform between isolates derived from a common ancestor or between isolates of nonmodel organisms. In addition, competition experiments require that competing strains be grown in the same environment, so they cannot be used to infer the fitness consequence o

SUBMITTER: Ram Y 

PROVIDER: S-EPMC6642348 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature