High-frequency microrheology in 3D reveals mismatch between cytoskeletal and extracellular matrix mechanics.

ABSTRACT: Mechanical homeostasis describes how cells sense physical cues from the microenvironment and concomitantly remodel both the cytoskeleton and the surrounding extracellular matrix (ECM). Such feedback is thought to be essential to healthy development and maintenance of tissue. However, the nature of the dynamic coupling between microscale cell and ECM mechanics remains poorly understood. Here we investigate how and whether cells remodel their cortex and basement membrane to adapt to their microenvironment. We measured both intracellular and extracellular viscoelasticity, generating a full factorial dataset on 5 cell lines in 2 ECMs subjected to 4 cytoskeletal drug treatments at 2 time points. Nonmalignant breast epithelial cells show a similar viscoelasticity to that measured for the local E

SUBMITTER: Staunton JR 

PROVIDER: S-EPMC6642418 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature