Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases.

ABSTRACT:

Background

Avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) are important avian pathogens that can cause enormous economic loss on the poultry industry. Different respiratory etiological agents may induce similar clinical signs that make differential diagnosis difficult. Importantly, AIV brings about severe threat to human public health. Therefore, a novel method that can distinguish these viruses quickly and simultaneously is urgently needed.

Results

In this study, an oligonucleotide microarray system was developed. AIV, including H5, H7, and H9 subtypes; NDV; and IBV were simultaneously detected and differentiated on a microarray. Three probes specific for AIV, NDV, and IBV, as well as three other probes for differentiating

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC6642548 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature