Absence of an embryonic stem cell DNA methylation signature in human cancer.

ABSTRACT:

Background

Differentiated cells that arise from stem cells in early development contain DNA methylation features that provide a memory trace of their fetal cell origin (FCO). The FCO signature was developed to estimate the proportion of cells in a mixture of cell types that are of fetal origin and are reminiscent of embryonic stem cell lineage. Here we implemented the FCO signature estimation method to compare the fraction of cells with the FCO signature in tumor tissues and their corresponding nontumor normal tissues.

Methods

We applied our FCO algorithm to discovery data sets obtained from The Cancer Genome Atlas (TCGA) and replication data sets obtained from the Gene Expression Omnibus (GEO) data repository. Wilcoxon rank sum tests, linear regression models with adjustmen

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC6642562 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature