Quantifying Argonaute 2 (Ago2) expression to stratify breast cancer.

ABSTRACT:

Background

Argonaute-2 (Ago2) is an essential component of microRNA biogenesis implicated in tumourigenesis. However Ago2 expression and localisation in breast cancer remains undetermined. The aim was to define Ago2 expression (mRNA and protein) and localisation in breast cancer, and investigate associations with clinicopathological details.

Methods

Ago2 protein was stained in breast cancer cell lines and tissue microarrays (TMAs), with intensity and localization assessed. Staining intensity was correlated with clinicopathological details. Using independent databases, Ago2 mRNA expression and gene alterations in breast cancer were investigated.

Results

In the breast cancer TMAs, 4 distinct staining intensities were observed (Negative, Weak, Moderate, Strong), with 64.

SUBMITTER: Casey MC 

PROVIDER: S-EPMC6642579 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature