Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT.

ABSTRACT:

Background

The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant's genomic and transcriptomic features. Most N. benthamiana accessions currently used in research lack proper documentation of their breeding history and provenance. One of these, the glycoengineered N. benthamiana line ΔXT/FT is increasingly used for the production of biopharmaceutical proteins.

Results

Based on an existing draft assembly of the N. benthamiana genome we predict 50,516 protein -encoding genes (62,216 transcripts) supported by expression data derived from 2.35 billion mRNA-seq reads. Using single-copy core genes we show high completeness of the pr

SUBMITTER: Schiavinato M 

PROVIDER: S-EPMC6642603 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature