The retinal environment induces microglia-like properties in recruited myeloid cells.

ABSTRACT:

Background

Microglia are essential to the development of the CNS and its homeostasis. Our prior findings suggested a niche model to describe the behaviors of retinal microglia. Here, we ask whether new myeloid cells recruited to the retina are constrained to resemble endogenous microglia morphologically and functionally.

Methods

Use of CD11c^DTR/GFP transgenic mouse allowed identification of two niches of retinal microglia distinguished by being GFP^lo or GFP^hi. We also used transgenic mice in which CX3CR1⁺ cells expressed YFP and were depletable following tamoxifen-induced expression of diphtheria toxin subunit A. We employed several ablation and injury stimulation protocols to examine the origin and fate of myeloid cells repopulati

SUBMITTER: McPherson SW 

PROVIDER: S-EPMC6642741 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature