Repair and translesion synthesis of O⁶-alkylguanine DNA lesions in human cells.

ABSTRACT: O⁶-alkyl-2'-deoxyguanosine (O⁶-alkyl-dG) lesions are among the most mutagenic and prevalent alkylated DNA lesions that are associated with cancer initiation and progression. In this study, using a shuttle vector-based strand-specific PCR-competitive replication and adduct bypass assay in conjunction with tandem MS for product identification, we systematically assessed the repair and replicative bypass of a series of O⁶-alkyl-dG lesions, with the alkyl group being a Me, Et, nPr, iPr, nBu, iBu, or sBu, in several human cell lines. We found that the extent of replication-blocking effects of these lesions is influenced by the size of the alkyl groups situated on the O⁶ position of the gua

SUBMITTER: Du H 

PROVIDER: S-EPMC6643039 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature