Reselection Yielding a Smaller and More Active Silver-Specific DNAzyme.

ABSTRACT: Ag10c is a recently reported RNA-cleaving DNAzyme obtained from in vitro selection. Its cleavage activity selectively requires Ag⁺ ions, and thus it has been used as a sensor for Ag⁺ detection. However, the previous selection yielded very limited information regarding its sequence requirement, since only ∼0.1% of the population in the final library were related to Ag10c and most other sequences were inactive. In this work, we performed a reselection by randomizing the 19 important nucleotides in Ag10c in such a way that a purine has an equal chance of being A or G, whereas a pyrimidine has an equal chance of being T or C. The round 3 library of the reselection was carefully analyzed and a statistic understanding of the relative importance of each nucleotide was obtain

SUBMITTER: Gu L 

PROVIDER: S-EPMC6643755 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature