An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila.

ABSTRACT: Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology, and physiology. However, the number of well-defined LexA enhancer trap insertions remains limited. In this study, we present the molecular characterization and initial tissue expression analysis of nearly 100 novel StanEx LexA enhancer traps, derived from the StanEx¹ index line. This includes 76 insertions into novel, distinct gene loci not previously associated with enhancer traps or targeted LexA constructs. Additionally, our studies revealed evidence for selective transposase-dependent replacement of a previously-undetected KP element on chromosome III within the StanEx¹ genetic backgr

SUBMITTER: Kockel L 

PROVIDER: S-EPMC6643891 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature