High-Resolution Asymmetrical Flow Field-Flow Fractionation Data Evaluation via Richardson-Lucy-Based Fractogram Correction.

ABSTRACT: Asymmetrical flow field-flow fractionation (AF4) is a chromatographic separation technique that can be used for a broad range of particles or macromolecules. As an orthogonal method to size exclusion chromatography (SEC) with a much broader separation size range (1-800 nm) AF4 is gaining importance. However, the data evaluation capacities are far behind in comparison to other techniques like analytical ultracentrifugation (AUC). A program for evaluation of data from AF4 with a coupled multiangle laser light scattering (MALLS) detector was developed that allows the determination of the distributions of diffusion coefficients ( D), hydrodynamic radii ( R_h), molecular weights ( M_w), and relative concentrations (RC) of the obtained species. In addition, two algorithms to

SUBMITTER: Schmid M 

PROVIDER: S-EPMC6643961 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature