Forster Resonance Energy-Transfer-Based Ratiometric Fluorescent Indicator for Quantifying Fluoride Ion in Water and Toothpaste.

ABSTRACT: A Förster resonance energy-transfer (FRET)-based ratiometric fluorescent indicator Cou-FITC-Si toward fluoride ion has been designed and synthesized by combining coumarin unit and fluorescein derivative as energy donor and acceptor, respectively. The fluorescein unit is capped with tert-butyldiphenylchlorosilane. The indicator gives out emission responses based on switch-on of the FRET process that triggered by the desilylation mediated by the fluoride ion. The fluorescence emission spectrum of Cou-FITC-Si presents a significant bathochromic shift of 59 nm after the addition of fluoride ion with up to 180-fold increase of the fluorescence intensity ratio. The limit of detection of the Cou-FITC-Si indicator system toward fluoride ion was estimated to be 3.3 ppb.

SUBMITTER: Qiu B 

PROVIDER: S-EPMC6644089 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature