Direct visualization of cardiac transcription factories reveals regulatory principles of nuclear architecture during pathological remodeling.

ABSTRACT: Heart failure is associated with hypertrophying of cardiomyocytes and changes in transcriptional activity. Studies from rapidly dividing cells in culture have suggested that transcription may be compartmentalized into factories within the nucleus, but this phenomenon has not been tested in vivo and the role of nuclear architecture in cardiac gene regulation is unknown. While alterations to transcription have been linked to disease, little is known about the regulation of the spatial organization of transcription and its properties in the pathological setting. In the present study, we investigate the structural features of endogenous transcription factories in the heart and determine the principles connecting chromatin structure to transcriptional regulation in vivo. Super-resolution imagin

SUBMITTER: Karbassi E 

PROVIDER: S-EPMC6644685 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature