Analysis of Acrolein-Derived 1, N²-Propanodeoxyguanosine Adducts in Human Lung DNA from Smokers and Nonsmokers.

ABSTRACT: Acrolein, the simplest α,β-unsaturated aldehyde, is present in relatively large quantities in cigarette smoke, and several studies have raised the possibility of it being a major etiological agent for smoking-related lung cancer. Acrolein reacts directly with DNA to form primarily Acr-dGuo adducts, which serve as important biomarkers for the assessment of exposure to acrolein and its potential role in smoking-related lung cancer. In this study, we developed an ultrasensitive and low-artifact method using liquid chromatography-nanoelectrospray ionization-high-resolution tandem mass spectrometry to quantitate Acr-dGuo adducts in normal lung tissue DNA obtained at surgery from lung cancer patients who never smoked and from those who continued smoking until surgery, as confirmed by urinary tot

SUBMITTER: Yang J 

PROVIDER: S-EPMC6644703 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature