Immobilization of Carbonic Anhydrase in Glass Micropipettes and Glass Fiber Filters for Flow-Through Reactor Applications.

ABSTRACT: There are various ways of immobilizing carbonic anhydrase (CA) on solid materials. One of the final aims is to apply immobilized CA for the catalytic hydration of carbon dioxide (CO₂) as a first step in the conversion of gaseous CO₂ into solid products. The immobilization method investigated allows a straightforward, stable, and quantifiable immobilization of bovine erythrocyte carbonic anhydrase (BCA) on silicate surfaces. The method is based on the use of a water-soluble, polycationic second-generation dendronized polymer with on average 1000 repeating units, abbreviated as de-PG2₁₀₀₀. Several copies of BCA were first covalently linked to de-PG2₁₀₀₀ through stable bisaryl hydrazone (BAH) bonds. Then, the de-PG2₁₀₀₀

SUBMITTER: Yoshimoto M 

PROVIDER: S-EPMC6645021 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature