Covalent Modification of Silicon Nitride Nanopore by Amphoteric Polylysine for Short DNA Detection.

ABSTRACT: In this work, we demonstrate a chemical modification approach, by means of covalent-bonding amphoteric poly-l-lysine (PLL) on the interior nanopore surface, which could intensively protect the pore from etching when exposed in the electrolyte under various pH conditions (from pH 4 to 12). Nanopore was generated via simple current dielectric breakdown methodology, covalent modification was performed in three steps, and the functional nanopore was fully characterized in terms of chemical structure, hydrophilicity, and surface morphology. I-V curves were recorded under a broad range of pH stimuli to evaluate the stability of the chemical bonding layer; the plotted curves demonstrated that nanopore with a covalent bonding layer has good pH tolerance and showed apparent reversibil

SUBMITTER: Yin B 

PROVIDER: S-EPMC6645049 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature