T Cell Repertoire Evolution after Allogeneic Bone Marrow Transplantation: An Organizational Perspective.

ABSTRACT: High-throughput sequencing (HTS) of human T cell receptors has revealed a high level of complexity in the T cell repertoire, which makes it difficult to correlate T cell reconstitution with clinical outcomes. The associations identified thus far are of a broadly statistical nature, precluding precise modeling of outcomes based on T cell repertoire development following bone marrow transplantation (BMT). Previous work has demonstrated an inherent, mathematically definable order observed in the T cells from a diverse group of donors, which is perturbed in recipients following BMT. In this study, T cell receptor (TCR)-β sequences from HLA-matched related donor and recipient pairs are analyzed to further develop this methodology. TCR-β sequencing from unsorted and sorted T cell subsets isolate

SUBMITTER: Meier JA 

PROVIDER: S-EPMC6645918 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature