Modified CLARITY Achieving Faster and Better Intact Mouse Brain Clearing and Immunostaining.

ABSTRACT: CLARITY is a hydrogel embedding clearing method that has the advantages of transparency, different tissue compatibility and immunostaining compatibility. However, there are also some limitations to CLARITY as it requires a long time to achieve transparency, and the electrophoresis clearing is complex. Therefore, we aimed to simplify the electrophoresis system and shorten the processing time of CLARITY. In our study, we developed a non-circulation electrophoresis system to achieve easier manipulation of electrophoresis clearing. We modified the original CLARITY protocol in hydrogel embedding methods, clearing buffer and immunostaining. When comparing brains processed by our modified method or the original protocol, we found our modifications permit faster and more efficient clearing and lab

SUBMITTER: Du H 

PROVIDER: S-EPMC6646319 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature