Improving production of N-glycosylated recombinant proteins by leaky Escherichia coli.

ABSTRACT: Escherichia coli has been considered as a promising host for the production of N-glycosylated therapeutic proteins and glycoconjugate vaccines. In this study, we developed a simple and efficient strategy for improving the production of N-glycosylated recombinant proteins by combining auto-induction with the use of a leaky E. coli strain. A leaky E. coli strain, designated as CLM37-Δlpp, was engineered by deleting the Braun's lipoprotein (lpp) gene of E. coli strain CLM37. Three distinct acceptor model N-glycosylated proteins, glyco-tagged human tenth fibronectin type III domain (FN3-Gly), enhanced green fluorescent protein (eGFP-Gly), and scFv of vascular endothelial growth factor receptor 3 (scFv-_VEGFR3-Gly) were then expressed in CLM37

SUBMITTER: Ding N 

PROVIDER: S-EPMC6646630 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature