Human Organoids Share Structural and Genetic Features with Primary Pancreatic Adenocarcinoma Tumors.

ABSTRACT: Patient-derived pancreatic ductal adenocarcinoma (PDAC) organoid systems show great promise for understanding the biological underpinnings of disease and advancing therapeutic precision medicine. Despite the increased use of organoids, the fidelity of molecular features, genetic heterogeneity, and drug response to the tumor of origin remain important unanswered questions limiting their utility. To address this gap in knowledge, primary tumor- and patient-derived xenograft (PDX)-derived organoids, and 2D cultures for in-depth genomic and histopathologic comparisons with the primary tumor were created. Histopathologic features and PDAC representative protein markers (e.g., claudin 4 and CA19-9) showed strong concordance. DNA- and RNA-sequencing (RNAseq) of single organoids revealed patient-s

SUBMITTER: Romero-Calvo I 

PROVIDER: S-EPMC6647028 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature