Increased PHGDH expression promotes aberrant melanin accumulation.

ABSTRACT:

Background

Copy number gain of the D-3-phosphoglycerate dehydrogenase (PHGDH) gene, which encodes the first enzyme in serine biosynthesis, is found in some human cancers including a subset of melanomas.

Methods

In order to study the effect of increased PHGDH expression in tissues in vivo, we generated mice harboring a PHGDH^tetO allele that allows tissue-specific, doxycycline-inducible PHGDH expression, and we analyzed the phenotype of mice with a ubiquitous increase in PHGDH expression.

Results

Tissues and cells derived from PHGDH^tetO mice exhibit increased serine biosynthesis. Histological examination of skin tissue from PHGDH^tetO mice reveals the presence of melanin granules in early anagen hair follicles, despite the fact that melan

SUBMITTER: Mattaini KR 

PROVIDER: S-EPMC6647269 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature